MANOJ SINGH*, MAYANK RAJ, RAJ SINGH, CHHAYA SINGH, SUSHIL KUMAR UPADHYAY, VANDANA SHARMA, JATINDER KUMAR SHARMA, YOGENDRA PRASAD PANDEY, DEEPAK YADAV AND VIKAS KUMAR
Department of Bio-Sciences and Technology, Maharishi Markandeshwar (Deemed to be University), Mullana,
Ambala-133 207 (Haryana), India
*(e-mail: manoj.singh@mmumullana.org; Mobile: 87070 11151)
(Redeived: December 30, 2025; Accepted: March 3, 2026)
ABSTRACT
The findings were firstly focused on the biomimetic synthesis of zinc oxide nanoparticles through aqueous leaf extract of Ocimum sanctum. The physico-chemical stability was achieved by optimizing various parameters. The absorption spectra at 400 nm were observed for zinc oxide nanoparticles. The atomic force microscopy analyzed the morphology of the nanocomposite, which was further confirmed through TEM micrograph. AFM analysis concluded that nanoparticles were non-agglomerated and polydispersed, as confirmed by two and three dimensional distribution views. The electron micrograph image disclosed that zinc oxide nanoparticles were polydispersed and dominantly as spherical with a size around 106 nm. The chemical reductions were further confirmed through FTIR analysis. The biogenic zinc oxide nanoparticles and their drug formulation showed profound antibacterial activity against pathogenic bacteria. The rich binding of zinc oxide nanoparticles showed potential medicinal effects that can be used to treat several harmful infectious diseases. Hence, plant based metal oxide nanoparticles met the demand for less toxic formulations during drug development and delivery.
Key words: Nanoparticles, antibacterial activity, biomimetic, electron microscope, optimization