MAYANK RAJ, MANOJ SINGH*, BABITA, RAJ SINGH AND SUSHIL KUMAR UPADHYAY
Department of Bio-Sciences & Technology, MMEC, Maharishi Markandeshwar (Deemed to be University), Mullana, Ambala-133 207 (Haryana), India
*(e-mail: manoj.singh@mmumullana.org; Mobile: 87070 11151)
(Received: June 25, 2025; Accepted: July 20, 2025)
ABSTRACT
The rise of multidrug-resistant (MDR) microbes is a significant and formidable public health challenge, garnering substantial attention from the scientific community. The current study attempted to manufacture silver nanoparticles utilizing the leaves of Aloe vera, a widely used medicinal herb. The bioreduced silver nanoparticles were characterized by UV-Vis spectrophotometer, dynamic light scattering (DLS), fourier transform infra-red (FTIR) spectroscopy, transmission electron microscopy (TEM) and energy-dispersive X-ray spectroscopy (EDX). The mean particle size of the produced NPs was around 80 nm and spherical in shape. A qualitative examination of the reducing potential of leaf extract was also performed, indicating the presence of a large amount of reducing entities. FTIR studies demonstrated that the AgNPs were stabilized by the extract’s eugenols, terpenes and other aromatic components. The described nanoparticles exhibited growth inhibitory effects on many strains, including gram-positive and gram-negative bacteria such as Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli. when analyzed using the Agar Well-diffusion method. A significant decrease in the zone of inhibition was seen upon the application of the drug-conjugated silver nanoparticle. The green synthesis strategy opened up the possibility of creating extremely stable Ag nanoparticles with strict particle shape/size distribution from various leaf extracts to develop improved anti-bacterial medicines.
Key words: Antibacterial activity, silver nanoparticles, characterization, optimization