S. RAVI KIRAN, D. SANDHYA DEEPIKA, Y. T. RAJESH BABU*, M. RAMESH CHOWDARY AND G. VINAY KUMAR
Department of Botany, Andhra University, Visakhapatnam- 530 003 (Andhra Pradesh), India
*(e-mail: baburajesh0999@gmail.com; Mobile: 63008 84860, 90527 96457)
(Received: July 5, 2023; Accepted: August 9, 2023)
ABSTRACT
Cancer is still a major public health challenge, accounting for one out of every six deaths. Compounds with antioxidant capabilities are especially interesting because they may have anticancer qualities by neutralizing free radicals that can cause DNA damage and contribute to cancer. The antioxidant capacities (DPPH and FRAP assays) and cytotoxic activities (MTT assay) of human adenocarcinoma cells (PC-3) and mouse fibroblast cells (L-929) of Prunus ceylanica were studied. Results revealed that the highest antioxidant activities were shown in the methanol bark extract (IC50 = 40.19±0.75 μg/ml), while using the DPPH method and the acetone bark extract (IC50 = 15.77±1.25 μg/ml) through the FRAP assay. Furthermore, the acetone bark extract exhibited the highest total phenolic content (447.61±7.17 mg GAE/g dry extract). Remarkably, the acetone bark extract demonstrated significant specificity against PC-3 cells (P < 0.005) with the lowest IC50 value (27.74±1.96 μg/ml) and minimal toxicity to L-929 cells (IC50=175.08±0.01 μg/ml). These results suggested a possible approach for the future development of a novel anticancer medication against lethal adenocarcinoma. Key words : Adenocarcinoma cell line, DPPH assay, fibroblast cell line, FRAP assay, MTT assay