ANDRIYANA SETYAWATI*, SAMANHUDI, SRI HARTATI, NIA GUSNIAR, TILMIIDZAH SALMA FATHIN AND JOKO PRIHANTO
Department of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret (UNS), Jl. Ir. Sutami 36A, Kentingan, Surakarta, 57126, Central Java, Indonesia
*(e-mail: andriyanasetyawati@staff.uns.ac.id; Mobile: 628529 3942727)
(Received: January 17, 2024; Accepted: March 15, 2024)
ABSTRACT
Orchid is an ornamental plant which is an essential commodity in the international trade market because of the beauty of its flowers. This study aimed at examining the effect of kinetin and tomato extract on orchid growth and obtain the best concentration for developing Dendrobium stratiotes orchids in vitro on 1/2 MS media. This research was conducted at the Laboratory of Plant Physiology and Biotechnology, Universitas Sebelas Maret Surakarta, from July to December 2022. The method used was a factorial, completely randomized design (CRD) with two factors, the first factor was the concentration of kinetin (0, 0.5, 1.0, 1.5, and 2.0 ppm) and the second factor was the concentration of tomato extract (0, 50, 100, 150 and 200 g/l). Each treatment was repeated in five replications. Data analysis was carried out by analysis of variance (ANOVA). The Duncan Multiple Range Test (DMRT) was used at the 5% for regression test. The results showed that the interaction of kinetin and tomato extract treatments significantly affected plant height, number of roots, number of leaves and fresh weight. Applying kinetin at two ppm significantly affected plant height, number of lateral shoots and fresh weight. The application of tomato extract 200 g/l considerably affected plant height and fresh weight.
Key words: Cultured plantlet, hormone, micro-propagation, ornamental plant, plant growth promotors