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India’s Functional Fungi: Evaluating the Antioxidant Activity of Methanolic Extracts of Native Medicinal and Edible Mushrooms

SHREYA CHAHAL* AND ANIL SINDHU
Department of Biotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal, Sonipat-131 039 (Haryana), India
*(e-mail: shreyachahal57@gmail.com; Mobile: 86858 27789)
(Received: June 16, 2025; Accepted: July 27, 2025)

ABSTRACT

Oxidative stress, caused by reactive oxygen species (ROS), compromises cellular integrity and contributes to various diseases. This research investigated the antioxidant potential of Indian cultivated fungi, including Ganoderma lucidum, Hericium erinaceus, Agaricus bisporus, Auricularia auricular-judae, Calocybe indica, Pleurotus citrinopileatus, Cordyceps militaris and Pleurotus djamor. In vitro antioxidant activities were assessed using ABTS and DPPH assays. Fourier-transform infrared (FTIR) spectroscopy was employed to chemically characterize the extracts and identify functional groups contributing to their bioactive profiles. Phytochemical analysis confirmed the presence of major constituents, including polyphenols, flavonoids and terpenoids. These indigenous mushrooms demonstrated strong antioxidant activity, effectively neutralizing ROS and mitigating oxidative damage. Their traditional relevance and bioactivity support their potential use in nutraceutical and pharmaceutical formulations.
Key words: Antioxidant activity, DPPH assay, ABTS assay, Indian cultivated fungi, medicinal mushrooms, reactive oxygen species