MAHA SAUD KHALAF*, HAWAZIN AHMED ABID AND RAFEA ZAIDAN MUKHLIF ALSUGMIANY
Department of Biology, College of Science, Tikrit University, Iraq
*(e-mail: maha.s.khalaf4410@st.tu.edu.iq; Mobile: + 964 77136 11993)
(Received: May 25, 2023; Accepted: June 29, 2023)
ABSTRACT
Three local isolates of Alternaria spp. were collected from wheat seed in Salah AL-Deen. These were morphologically identified confirmed as Alternaria alternata, A. tenuissima and A. saponaria on the basis of internal transcribed spacer (ITS) sequence similarity by using two primers ITS-1 ITS-4 synthesized on the basis of conserved regions of the eukaryotic rRNA gene. The studied local isolates were compared with the global isolates preserved in the Genbank (NCBI). The wheat seed samples studied in this study were contaminated with different percentage of fungi. Alternaria spp. is post-harvested contaminated fungi. Therefore, the Silos will not receive the yield unless it meets the conditions for good storage. The molecular study confirmed the morphological diagnosis of Alternaria spp. Thus, the selected species showed genetic relationship by using DNA sequencing. Further, the bs showed a base pair which indicated that studied isolates belonged to Alternaria spp.
Key words : Alternaria, wheat, ITS sequence, Genbank, wheat