POONAM DHANDA, SUBHASH KAJLA AND PRIYANKA SIWACH*
Department of Biotechnology, Chaudhary Devi Lal University, Sirsa-125 055 (Haryana), India
*(e-mail : psiwach29@gmail.com; Mobile : 94669 15002)
(Received : May 5, 2022; Accepted : May 28, 2022)
ABSTRACT
Chlorophytum borivilianum (Safed Musli) is among the most important medicinal herbs. The present research
involves in vitro sterilization and regeneration from basal plate explant for direct shoot regeneration.
The basal plates were excised from the field from grown matured plants, sterilized with different sterilizing
agents with varying concentrations and time intervals followed by inoculation on the Murashige and
Skoog’s medium fortified with different concentrations of hormones. The sterilization treatment of
explants with 0.1% mercuric chloride (HgCl2) for 7 min along with 0.4% streptocyclin and 0.4% bavistin
for 120 min was proved to be best with maximum survival of 90% among all the treatments used. The MS
medium supplemented with 2.0 mg/l6-benzyl aminopurine (BAP) was found to be the most effective
media for shoot induction in safed musli with maximum 80% regeneration after 28 days of inoculation
producing 2.9 average shoots per explant.
Key words : Chlorophytum borivilianum, in vitro, sterilization, fortified, medicinal