ASHMITA CHHIKARA, PREETY ROHILLA AND PUSHPA DAHIYA*
Department of Botany, Maharshi Dayanand University, Rohtak-124 001 (Haryana), India
*(e-mail: puspha.dahiya@hotmail.com; Mobile: 94168 63575)
(Received: June 11, 2023; Accepted: July 19, 2023)
ABSTRACT
The demand of in use medications and the urge to search for new storehouse of bioactive compounds have led the scientists to explore more plants for their therapeutic potential. The present investigation was planned to assess qualitative as well as quantitative phyto-compounds of Sphagneticola trilobata extracts prepared in aqueous, methanol, chloroform and petroleum ether solvent. Furthermore, antibacterial efficacy of all four plant extracts was assessed against seven bacterial strains i.e. Bacillus subtilis (MTCC-2057), Chromobacterium violaceum (MTCC-2656), Escherichia coli (MTCC-41), Klebsiella pneumoniae (MTCC-109), Mycobacterium smegmatis (MTCC-992), Pseudomonas aeruginosa (MTCC-2453) and Staphylococcus aureus (MTCC-96) employing disc diffusion method. MIC was also estimated for the plant extracts using micro-broth dilution assay. The methanol extract showed the highest percentage yield and presence of maximum number of phytoconstituents in addition to maximum TPC and TFC. Splendid antibacterial potential of S. trilobata was recorded due to its inhibitory activity against two bacterial strains i.e. M. smegmatis and P. aeruginosa where ampicillin was found ineffective. This shows the vast antibacterial potential of the plant against ampicillin resistant strains. These promising results showed the great potential of this plant in pharmaceutical industries.
Key words : Antibacterial, Sphagneticola trilobata, phytoconstituents, percentage yield, MIC