ANUPMA MALIK, MANOJ KUMAR, SULEKHA CHAHAL, POONAM YADAV, SUDHIR KUMAR KATARIA AND SUNITA DALAL*
Department of Biotechnology, Kurukshetra University, Kurukshetra-136 119 (Haryana), India
*(e-mail : sdalal@kuk.ac.in; Mobile : 9812001469)
(Received : August 19, 2021; Accepted : October 10, 2021)
ABSTRACT
In vitro resistant profiling study of 11 antibiotics (ciprofloxacin, tetracycline, trimethoprim, ampicillin,
chloramphenicol, norfloxacin, erythromycin, nitrofurantoin, cefixime, amikacin and lincomycin) was
done against five gram-positive and three gram-negative bacterial strains. All strains were found
multidrug resistant. Pseudomonas aeruginosa was found to be Extended Spectrum Lactamase (ESBL)
producer. Ten phytochemicals (gallic acid, jasmonic acid, piperine, quercetin, rutin, salicyclic acid,
shikimic acid, berberine and tannic acid) were evaluated for antibacterial activity. Only rutin
demonstrated the potential antibacterial activity. But in the synergistic studies, all 10 phytochemicals
with various antibiotics revealed that gallic acid, salicyclic acid, berberin, rutin and quercetin
complimented strong inhibitory potential with chloramphenicol, tetracycline and erythromycin. The
study validated potential of phytochemicals as genuine source of antibiotic resistance modifying
agents.
Key words : Phytochemical, antibiotics, ESBL, bacteria, antibiotic resistant