SUREKHA MANHAS, ARTI DEVI* AND ZAVED AHMED KHAN
Department of Biotechnology, University Institute of Engineering, Chandigarh University, H-05, Ludhiana- Chandigarh State Highway, Sahibzada Ajit Singh Nagar-140 413 (Punjab), India
*(e-mail: arti.e6031@cumail.in; Mobile: 78140 37122)
(Received: August 13, 2024; Accepted: October 20, 2024)
ABSTRACT
This research focused on the in vitro evaluation of the hypoglycemic and antioxidant effects of leaf extracts of Camellia sinensis using ethanol, methanol and water. The assessment techniques employed included -amylase, -glucosidase inhibitory assays, glucose adsorption, glucose diffusion methods, glucose uptake potential in yeast cells, as well as DPPH, hydrogen peroxide activity and ferric reducing power radical scavenging activity. The green tea extracts demonstrated significant antioxidant activity by eliminating free radicals: 65-90.21% (DPPH), 60-80% (HO2O2) and 55-76% (FRAP). Although the in vitro glucose diffusion retardation, glucose uptake capacity and glucose adsorption suggested potential therapeutic value for green tea, further research is necessary to identify the relevant bioactive compounds and to validate their pharmacological properties in vivo.
Key words: Oxidative stress, diabetes, epigallocatechin-3-gallate, antioxidant, oxidative damage