P. JANAKI RAO, J. RAMALAKSHMANA, Y. T. RAJESH BABU* AND S. B. PADAL
Department of Botany, Andhra University, Visakhapatnam-530 003 (Andhra Pradesh), India *(e-mail: baburajesh0999@gmail.com; Mobile: 63008 84860, 90527 96457)
(Received: November 30, 2024; Accepted: January 20, 2025)
ABSTRACT
In present study, various solvent extracts of Cymbidium aloifolium were analyzed for their phytochemical profiles, and antimicrobial and antioxidant activities. The antimicrobial activity was tested using the agar well diffusion method against four pathogens: Bacillus subtilis, Staphylococcus aureus, Pseudomonas fluorescens and Streptococcus mutans. The antioxidant activity was evaluated using the DPPH scavenging assay. Additionally, GCMS analysis was conducted to determine the chemical composition of the extracts.The methanolic extract demonstrated the highest antioxidant activity, with an IC50 value of 20.45±1.02 μg/ml, whereas the acetone extract showed the lowest antioxidant activity, with an IC50 value of 74.82±1.64 μg/ml. Methanol extracts also exhibited strong antibacterial activities, with inhibition zones ranging from 6.3±0.57 to 18.3±0.57 mm, while the aqueous extracts showed no antimicrobial activity. GCMS analysis of the methanolic extract of C. aloifolium identified nine components, with 1,3- Benzenedicarboxylic acid, bis (2-ethylhexyl) ester (49.19%) and γ -Sitosterol (19.89%) being the most prominent. These findings suggest that the methanol extract of C. aloifolium leaves is a rich source of bioactive compounds with notable antioxidant and antimicrobial properties, making it a potential source for the development of treatments for various diseases.
Key words: Phytochemicals, DPPH assay, GCMS analysis, antioxidant activities